af4 af2000 mt Search Results


af2000 mt series mid temperature  (Postnova Analytics)
90
Postnova Analytics af2000 mt series mid temperature
Af2000 Mt Series Mid Temperature, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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af20 0 0 mt instrument  (Postnova Analytics)
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Postnova Analytics af20 0 0 mt instrument
Af20 0 0 Mt Instrument, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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af20 0 0 mt instrument - by Bioz Stars, 2026-03
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mals detector  (Brookhaven Instruments)
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Brookhaven Instruments mals detector
Mals Detector, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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on-line uv-vis detector  (Postnova Analytics)
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Postnova Analytics on-line uv-vis detector
On Line Uv Vis Detector, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eclipse neon  (Waters Corporation)
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Waters Corporation eclipse neon
<t>AF4</t> purification of siRNA molecules using Eclipse <t>NEON</t> multidetector system (Wyatt). A 10 kDa membrane, 400 µm channel height, and 4 mL/min cross-flow velocity were used. ( A ) A representative fractogram for the AF4 purification of siRNA swarm obtained after digestion of phi6 dsRNA with Giardia Dicer (black line). Cross-flow gradient from 4 mL/min to 0.1 mL/min is shown with black dashed line (right y -axis). UV detector response at 260 nm is shown in volts (V) (left y -axis). Time axis shows the UV fractogram from the beginning of elution program excluding the focusing time. ( B ) The fractions collected for 2 min (1 mL) from the elution step were analyzed in 4% ( w / v ) Nusieve CTG agarose gel. IP refers to the input sample. DL is Ultra low range DNA ladder and M20 is a No Limit 20 bp DNA fragment (Fermentas). Mobility of the selected dsDNA molecules (in bp) is indicated on the left. ( C ) Molar mass of siRNAs was calculated from light scattering and concentration data with ASTRA 8.0 software. The sample peak obtained with dRI detector is presented. The molar mass distribution is shown with a horizontal line.
Eclipse Neon, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eclipse neon/product/Waters Corporation
Average 93 stars, based on 1 article reviews
eclipse neon - by Bioz Stars, 2026-03
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tip and focus pump  (Postnova Analytics)
90
Postnova Analytics tip and focus pump
<t>AF4</t> purification of siRNA molecules using Eclipse <t>NEON</t> multidetector system (Wyatt). A 10 kDa membrane, 400 µm channel height, and 4 mL/min cross-flow velocity were used. ( A ) A representative fractogram for the AF4 purification of siRNA swarm obtained after digestion of phi6 dsRNA with Giardia Dicer (black line). Cross-flow gradient from 4 mL/min to 0.1 mL/min is shown with black dashed line (right y -axis). UV detector response at 260 nm is shown in volts (V) (left y -axis). Time axis shows the UV fractogram from the beginning of elution program excluding the focusing time. ( B ) The fractions collected for 2 min (1 mL) from the elution step were analyzed in 4% ( w / v ) Nusieve CTG agarose gel. IP refers to the input sample. DL is Ultra low range DNA ladder and M20 is a No Limit 20 bp DNA fragment (Fermentas). Mobility of the selected dsDNA molecules (in bp) is indicated on the left. ( C ) Molar mass of siRNAs was calculated from light scattering and concentration data with ASTRA 8.0 software. The sample peak obtained with dRI detector is presented. The molar mass distribution is shown with a horizontal line.
Tip And Focus Pump, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tip and focus pump/product/Postnova Analytics
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autosampler  (Postnova Analytics)
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Postnova Analytics autosampler
<t>AF4</t> purification of siRNA molecules using Eclipse <t>NEON</t> multidetector system (Wyatt). A 10 kDa membrane, 400 µm channel height, and 4 mL/min cross-flow velocity were used. ( A ) A representative fractogram for the AF4 purification of siRNA swarm obtained after digestion of phi6 dsRNA with Giardia Dicer (black line). Cross-flow gradient from 4 mL/min to 0.1 mL/min is shown with black dashed line (right y -axis). UV detector response at 260 nm is shown in volts (V) (left y -axis). Time axis shows the UV fractogram from the beginning of elution program excluding the focusing time. ( B ) The fractions collected for 2 min (1 mL) from the elution step were analyzed in 4% ( w / v ) Nusieve CTG agarose gel. IP refers to the input sample. DL is Ultra low range DNA ladder and M20 is a No Limit 20 bp DNA fragment (Fermentas). Mobility of the selected dsDNA molecules (in bp) is indicated on the left. ( C ) Molar mass of siRNAs was calculated from light scattering and concentration data with ASTRA 8.0 software. The sample peak obtained with dRI detector is presented. The molar mass distribution is shown with a horizontal line.
Autosampler, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/autosampler/product/Postnova Analytics
Average 90 stars, based on 1 article reviews
autosampler - by Bioz Stars, 2026-03
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teflon spacer  (Postnova Analytics)
90
Postnova Analytics teflon spacer
<t>AF4</t> purification of siRNA molecules using Eclipse <t>NEON</t> multidetector system (Wyatt). A 10 kDa membrane, 400 µm channel height, and 4 mL/min cross-flow velocity were used. ( A ) A representative fractogram for the AF4 purification of siRNA swarm obtained after digestion of phi6 dsRNA with Giardia Dicer (black line). Cross-flow gradient from 4 mL/min to 0.1 mL/min is shown with black dashed line (right y -axis). UV detector response at 260 nm is shown in volts (V) (left y -axis). Time axis shows the UV fractogram from the beginning of elution program excluding the focusing time. ( B ) The fractions collected for 2 min (1 mL) from the elution step were analyzed in 4% ( w / v ) Nusieve CTG agarose gel. IP refers to the input sample. DL is Ultra low range DNA ladder and M20 is a No Limit 20 bp DNA fragment (Fermentas). Mobility of the selected dsDNA molecules (in bp) is indicated on the left. ( C ) Molar mass of siRNAs was calculated from light scattering and concentration data with ASTRA 8.0 software. The sample peak obtained with dRI detector is presented. The molar mass distribution is shown with a horizontal line.
Teflon Spacer, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellulose membrane  (Postnova Analytics)
90
Postnova Analytics cellulose membrane
<t>AF4</t> purification of siRNA molecules using Eclipse <t>NEON</t> multidetector system (Wyatt). A 10 kDa membrane, 400 µm channel height, and 4 mL/min cross-flow velocity were used. ( A ) A representative fractogram for the AF4 purification of siRNA swarm obtained after digestion of phi6 dsRNA with Giardia Dicer (black line). Cross-flow gradient from 4 mL/min to 0.1 mL/min is shown with black dashed line (right y -axis). UV detector response at 260 nm is shown in volts (V) (left y -axis). Time axis shows the UV fractogram from the beginning of elution program excluding the focusing time. ( B ) The fractions collected for 2 min (1 mL) from the elution step were analyzed in 4% ( w / v ) Nusieve CTG agarose gel. IP refers to the input sample. DL is Ultra low range DNA ladder and M20 is a No Limit 20 bp DNA fragment (Fermentas). Mobility of the selected dsDNA molecules (in bp) is indicated on the left. ( C ) Molar mass of siRNAs was calculated from light scattering and concentration data with ASTRA 8.0 software. The sample peak obtained with dRI detector is presented. The molar mass distribution is shown with a horizontal line.
Cellulose Membrane, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellulose membrane - by Bioz Stars, 2026-03
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multi-angle light scattering (malls  (Postnova Analytics)
90
Postnova Analytics multi-angle light scattering (malls
<t>AF4</t> purification of siRNA molecules using Eclipse <t>NEON</t> multidetector system (Wyatt). A 10 kDa membrane, 400 µm channel height, and 4 mL/min cross-flow velocity were used. ( A ) A representative fractogram for the AF4 purification of siRNA swarm obtained after digestion of phi6 dsRNA with Giardia Dicer (black line). Cross-flow gradient from 4 mL/min to 0.1 mL/min is shown with black dashed line (right y -axis). UV detector response at 260 nm is shown in volts (V) (left y -axis). Time axis shows the UV fractogram from the beginning of elution program excluding the focusing time. ( B ) The fractions collected for 2 min (1 mL) from the elution step were analyzed in 4% ( w / v ) Nusieve CTG agarose gel. IP refers to the input sample. DL is Ultra low range DNA ladder and M20 is a No Limit 20 bp DNA fragment (Fermentas). Mobility of the selected dsDNA molecules (in bp) is indicated on the left. ( C ) Molar mass of siRNAs was calculated from light scattering and concentration data with ASTRA 8.0 software. The sample peak obtained with dRI detector is presented. The molar mass distribution is shown with a horizontal line.
Multi Angle Light Scattering (Malls, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mals instrument  (Brookhaven Instruments)
90
Brookhaven Instruments mals instrument
<t>AF4</t> purification of siRNA molecules using Eclipse <t>NEON</t> multidetector system (Wyatt). A 10 kDa membrane, 400 µm channel height, and 4 mL/min cross-flow velocity were used. ( A ) A representative fractogram for the AF4 purification of siRNA swarm obtained after digestion of phi6 dsRNA with Giardia Dicer (black line). Cross-flow gradient from 4 mL/min to 0.1 mL/min is shown with black dashed line (right y -axis). UV detector response at 260 nm is shown in volts (V) (left y -axis). Time axis shows the UV fractogram from the beginning of elution program excluding the focusing time. ( B ) The fractions collected for 2 min (1 mL) from the elution step were analyzed in 4% ( w / v ) Nusieve CTG agarose gel. IP refers to the input sample. DL is Ultra low range DNA ladder and M20 is a No Limit 20 bp DNA fragment (Fermentas). Mobility of the selected dsDNA molecules (in bp) is indicated on the left. ( C ) Molar mass of siRNAs was calculated from light scattering and concentration data with ASTRA 8.0 software. The sample peak obtained with dRI detector is presented. The molar mass distribution is shown with a horizontal line.
Mals Instrument, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mals instrument/product/Brookhaven Instruments
Average 90 stars, based on 1 article reviews
mals instrument - by Bioz Stars, 2026-03
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cellulose membrane (rc, cut-off: 10 kda  (Postnova Analytics)
90
Postnova Analytics cellulose membrane (rc, cut-off: 10 kda
<t>AF4</t> purification of siRNA molecules using Eclipse <t>NEON</t> multidetector system (Wyatt). A 10 kDa membrane, 400 µm channel height, and 4 mL/min cross-flow velocity were used. ( A ) A representative fractogram for the AF4 purification of siRNA swarm obtained after digestion of phi6 dsRNA with Giardia Dicer (black line). Cross-flow gradient from 4 mL/min to 0.1 mL/min is shown with black dashed line (right y -axis). UV detector response at 260 nm is shown in volts (V) (left y -axis). Time axis shows the UV fractogram from the beginning of elution program excluding the focusing time. ( B ) The fractions collected for 2 min (1 mL) from the elution step were analyzed in 4% ( w / v ) Nusieve CTG agarose gel. IP refers to the input sample. DL is Ultra low range DNA ladder and M20 is a No Limit 20 bp DNA fragment (Fermentas). Mobility of the selected dsDNA molecules (in bp) is indicated on the left. ( C ) Molar mass of siRNAs was calculated from light scattering and concentration data with ASTRA 8.0 software. The sample peak obtained with dRI detector is presented. The molar mass distribution is shown with a horizontal line.
Cellulose Membrane (Rc, Cut Off: 10 Kda, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellulose membrane (rc, cut-off: 10 kda/product/Postnova Analytics
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Image Search Results


AF4 purification of siRNA molecules using Eclipse NEON multidetector system (Wyatt). A 10 kDa membrane, 400 µm channel height, and 4 mL/min cross-flow velocity were used. ( A ) A representative fractogram for the AF4 purification of siRNA swarm obtained after digestion of phi6 dsRNA with Giardia Dicer (black line). Cross-flow gradient from 4 mL/min to 0.1 mL/min is shown with black dashed line (right y -axis). UV detector response at 260 nm is shown in volts (V) (left y -axis). Time axis shows the UV fractogram from the beginning of elution program excluding the focusing time. ( B ) The fractions collected for 2 min (1 mL) from the elution step were analyzed in 4% ( w / v ) Nusieve CTG agarose gel. IP refers to the input sample. DL is Ultra low range DNA ladder and M20 is a No Limit 20 bp DNA fragment (Fermentas). Mobility of the selected dsDNA molecules (in bp) is indicated on the left. ( C ) Molar mass of siRNAs was calculated from light scattering and concentration data with ASTRA 8.0 software. The sample peak obtained with dRI detector is presented. The molar mass distribution is shown with a horizontal line.

Journal: Pharmaceuticals

Article Title: Native RNA Purification Method for Small RNA Molecules Based on Asymmetrical Flow Field-Flow Fractionation

doi: 10.3390/ph15020261

Figure Lengend Snippet: AF4 purification of siRNA molecules using Eclipse NEON multidetector system (Wyatt). A 10 kDa membrane, 400 µm channel height, and 4 mL/min cross-flow velocity were used. ( A ) A representative fractogram for the AF4 purification of siRNA swarm obtained after digestion of phi6 dsRNA with Giardia Dicer (black line). Cross-flow gradient from 4 mL/min to 0.1 mL/min is shown with black dashed line (right y -axis). UV detector response at 260 nm is shown in volts (V) (left y -axis). Time axis shows the UV fractogram from the beginning of elution program excluding the focusing time. ( B ) The fractions collected for 2 min (1 mL) from the elution step were analyzed in 4% ( w / v ) Nusieve CTG agarose gel. IP refers to the input sample. DL is Ultra low range DNA ladder and M20 is a No Limit 20 bp DNA fragment (Fermentas). Mobility of the selected dsDNA molecules (in bp) is indicated on the left. ( C ) Molar mass of siRNAs was calculated from light scattering and concentration data with ASTRA 8.0 software. The sample peak obtained with dRI detector is presented. The molar mass distribution is shown with a horizontal line.

Article Snippet: In our experiments, we used two AF4 instruments currently available on the market, AF2000 MT (Postnova Analytics) and Eclipse NEON (Wyatt Technology).

Techniques: Purification, Membrane, Agarose Gel Electrophoresis, Concentration Assay, Software